Concentration Assay:Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions.
Article Snippet: .. THP1 cells, at a concentration of 1 × 105 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4, Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions
Article Snippet: .. THP1 cells, at a concentration of 1 × 10 5 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4 , Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Incubation:Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions.
Article Snippet: .. THP1 cells, at a concentration of 1 × 105 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4, Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions
Article Snippet: .. THP1 cells, at a concentration of 1 × 10 5 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4 , Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Positive Control:Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions.
Article Snippet: .. THP1 cells, at a concentration of 1 × 105 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4, Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions
Article Snippet: .. THP1 cells, at a concentration of 1 × 10 5 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4 , Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Control:Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions.
Article Snippet: .. THP1 cells, at a concentration of 1 × 105 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4, Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions
Article Snippet: .. THP1 cells, at a concentration of 1 × 10 5 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4 , Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Capsules:Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions.
Article Snippet: .. THP1 cells, at a concentration of 1 × 105 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4, Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Article Title: Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions
Article Snippet: .. THP1 cells, at a concentration of 1 × 10 5 cells per well in a 96-well plate, were incubated for 24 h with either KRH buffer, 10 ng/mL lipopolysaccharides (LPS from E. coli O111:B4 , Invivogen, France, cat#tlrl-3pelps) as a positive control, or varying amounts of empty microcapsules (5, 10, or 20 control alginate capsules or alginate with ECM capsules). .. After incubation, 20 μL of culture supernatant was transferred to a new 96-well plate and mixed with 180 μL of QUANTI-BlueTM Solution (InvivoGen, France).
Cell Culture:Article Title: NLRP3 inhibition by VTX3232 tempers inflammation resulting in reduced body weight, hyperglycemia, and hepatic steatosis in obese male mice
Article Snippet: Chromatographic separation was achieved on a Waters ACQUITY UPLC CSH C18 column (1.7 μm, 2.1 × 50 mm) at room temperature using a gradient mobile phase system of water and acetonitrile (both with 0.1% formic acid) at 0.6 mL/min flow rate over 2.5 min. Mass spectrometry detection employed positive ionization mode with MRM transitions of 271.10 → 91.10 for tolbutamide (internal standard) and 382.20 → 255.20 for VTX3232, with optimized source parameters and a 2.0 μL injection volume. .. THP1 cells (Invivogen, thpd-nfis; purchased as mycoplasma-free THP1 cells and regularly tested for mycoplasma) were cultured in RPMI-1640 GlutaMAX (Gibco, 72400047) supplemented with 10 % FBS (Gibco, A5256801), 1x penicillin/streptomycin (Gibco, 15140122), 100 μg/mL Normocin (InvivoGen, ant-nr-1), 10 μg/mL Blasticidin S (Gibco, A11139-03), and 100 μg/mL Zeocin (InvivoGen, ant-zn-05). ..
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