thp1 dualtm human monocyte cell line  (InvivoGen)

Journal: Communications Biology

Article Title: Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states

doi: 10.1038/s42003-025-09343-7

Figure Lengend Snippet: a THP1 cell states and treatments. b Sample overview for transcriptome analysis. c Organization of samples into seven subgroups by unsupervised clustering as shown in Supplementary Fig. . Numbers in parentheses denote the total number of samples included in the DGE analysis for each subgroup. * denotes outlier samples that did not cluster with their expected treatment group. d Differentially regulated gene from comparing responses to the various treatments. A total of 1214 upregulated genes in monocytes under various treatments were selected for further analysis as shown in Fig. . Dig. digitonin, Lenti OE lentiviral OE of the indicated protein.

Article Snippet: THP1 cells from ATCC TIB-202 were cultured in Gibco RPMI 1640 supplemented with Sigma-Aldrich 10% v/v heat-inactivated fetal bovine serum (FBS), 100 U ml −1 penicillin, 100 μg ml −1 streptomycin, and 2 mM L-glutamine.

Techniques:

Journal: Communications Biology

Article Title: Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states

doi: 10.1038/s42003-025-09343-7

Figure Lengend Snippet: a Heatmaps displaying either absolute abundance (TPM, left panel) or row-normalized differential expression (z-score, right panel) of upregulated genes under various treatments in parental THP1 monocytes. Genes are grouped based on their unique or overlapping regulation across treatments as labeled on the left side of the heatmap. Genes assigned to various groups are listed in Supplementary Data . Each row corresponds to a gene, while each column represents a sample, either untreated or treated with IFNB1 protein, digitonin, digitonin with cGAMP, or LPS. Genes within each group are sorted by decreasing abundance. Numbers in parentheses denote the total number of genes identified per group. Representative genes are indicated by name. The transcript abundance scale also applies to subsequent figures. Dig. digitonin, TPM transcripts per million. b Expression levels (TPM) of representative genes from each gene group in parental THP1 monocytes. Each data point represents the TPM value for an individual sample, as listed in Supplementary Data , with the mean indicated by the bar.

Article Snippet: THP1 cells from ATCC TIB-202 were cultured in Gibco RPMI 1640 supplemented with Sigma-Aldrich 10% v/v heat-inactivated fetal bovine serum (FBS), 100 U ml −1 penicillin, 100 μg ml −1 streptomycin, and 2 mM L-glutamine.

Techniques: Quantitative Proteomics, Labeling, Expressing

Journal: Communications Biology

Article Title: Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states

doi: 10.1038/s42003-025-09343-7

Figure Lengend Snippet: a Group I genes uniquely induced by cGAMP in wild-type THP1 are impacted by IRF KO or OE. b A selected subset of Group I genes demonstrating distinct IRF3- or IRF7-dependent induction in response to cGAMP treatment. A total of 88 Group I genes (log 2 FC > 2, TPM > 20) were evaluated, and those showing the following fold induction threshold criteria in OE samples are shown: IRF3-dependent (log 2 FC > 2 in IRF3 OE, log 2 FC < 2 in IRF7 OE), IRF3- or IRF7-dependent (log 2 FC > 2 in IRF3 or IRF7 OE), and IRF7-dependent (log 2 FC > 2 in IRF7 OE, log 2 FC < 2 in IRF3 OE). Genes uniquely or commonly upregulated by IRF3 or IRF7 are indicated. Only 25 of the 45 identified IRF3-dependent genes are arbitrarily selected and displayed for simplicity. Dig. digitonin, Lenti OE lentiviral OE of the indicated protein.

Article Snippet: THP1 cells from ATCC TIB-202 were cultured in Gibco RPMI 1640 supplemented with Sigma-Aldrich 10% v/v heat-inactivated fetal bovine serum (FBS), 100 U ml −1 penicillin, 100 μg ml −1 streptomycin, and 2 mM L-glutamine.

Techniques:

Journal: Communications Biology

Article Title: Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states

doi: 10.1038/s42003-025-09343-7

Figure Lengend Snippet: a All Group II ISGs required IFNAR2 for induction by IFNB1 protein in the absence of cGAMP. Group IIb genes representing ISGs induced by IFNB1 protein or cGAMP in wild-type THP1 were impacted by IFNAR2 KO as well as IRF KO and OE. A subset of 29 Group IIb ISGs (IIRGs) also inducible by IRF3, retained strong induction (log 2 FC > 2) in IFNAR2 KO sample following cGAMP treatment and are marked with a black side bar. b IIRGs are shown in expanded view. IRF3 or IRF7 OE in KO samples rescued their induction following cGAMP treatment.

Article Snippet: THP1 cells from ATCC TIB-202 were cultured in Gibco RPMI 1640 supplemented with Sigma-Aldrich 10% v/v heat-inactivated fetal bovine serum (FBS), 100 U ml −1 penicillin, 100 μg ml −1 streptomycin, and 2 mM L-glutamine.

Techniques:

Journal: Communications Biology

Article Title: Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states

doi: 10.1038/s42003-025-09343-7

Figure Lengend Snippet: Group IVa genes exclusively induced by LPS and Group IVb genes primarily induced by LPS genes with minor co-induction by digitonin in wild-type and various KO THP1 monocytes. Induction of these genes remained unaffected by the KO of IRF or IFNAR2. Representative key pro-inflammatory genes are indicated by name.

Article Snippet: THP1 cells from ATCC TIB-202 were cultured in Gibco RPMI 1640 supplemented with Sigma-Aldrich 10% v/v heat-inactivated fetal bovine serum (FBS), 100 U ml −1 penicillin, 100 μg ml −1 streptomycin, and 2 mM L-glutamine.

Techniques:

Journal: Communications Biology

Article Title: Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states

doi: 10.1038/s42003-025-09343-7

Figure Lengend Snippet: a Group V genes co-induced by cGAMP or LPS in wild-type THP1 cells were impacted by IRF KO and OE. Their induction depended on IRF3 in cGAMP-treated samples but was IRF3-independent in LPS-treated samples. b Group VI genes co-induced by cGAMP, IFNB1 protein, or LPS in wild-type THP1 were impacted by IFNAR2 KO as well as IRF KO and OE. IFNAR2 was required for IFNB1-protein-induced activation but was dispensable in cGAMP-induced activation. IRF3 OE restored activation in response to cGAMP.

Article Snippet: THP1 cells from ATCC TIB-202 were cultured in Gibco RPMI 1640 supplemented with Sigma-Aldrich 10% v/v heat-inactivated fetal bovine serum (FBS), 100 U ml −1 penicillin, 100 μg ml −1 streptomycin, and 2 mM L-glutamine.

Techniques: Activation Assay

Journal: Communications Biology

Article Title: Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states

doi: 10.1038/s42003-025-09343-7

Figure Lengend Snippet: 283 genes upregulated by LPS in THP1 macrophages showing overlapping induction by various treatments in THP1 monocytes (Fig. ). Numbers in parentheses denote the total number of overlapping genes identified per group. Several Group I and IIb genes are also induced by LPS in THP1 macrophages, including IFNB1 and multiple ISGs; their induction is IRF3-dependent.

Article Snippet: THP1 cells from ATCC TIB-202 were cultured in Gibco RPMI 1640 supplemented with Sigma-Aldrich 10% v/v heat-inactivated fetal bovine serum (FBS), 100 U ml −1 penicillin, 100 μg ml −1 streptomycin, and 2 mM L-glutamine.

Techniques: